Fig. 2

ZL170 reduces TNBC cell migration, invasion and proliferation, but does not induce apoptosis in vitro. a Cell viability of MDA-MB-231, 4 T1 and PyMT cells treated with vehicle or 20 μM ZL170 for 24 h (n = 3) (b, d and f) Boyden chamber migration and invasion assays of MDA-MB-231 (b), 4 T1 (d) and PyMT (f) cells (n = 3). Cells pretreated with ZL170 at different doses for 24 h were seeded in upper insert in the presence (for invasion assay) or absence (for migration assay) of pre-coated Matrigel. c, e and g Quantification of the migrated or invaded cells as shown in c, e and g, respectively. h Migration wound healing assay of MDA-MB-231 and 4 T1 cells in the presence of different doses of ZL170. i Quantification of the wound healing as shown in h. j Cell viability of MDA-MB-231, 4 T1 and PyMT cells treated with vehicle or different doses of ZL170 for 48 h (n = 3). k Cell apoptosis analysis of MDA-MB-231 cells that were treated with vehicle or different doses of ZL170 for 48 h (n = 3). Cells were co-stained with Annexin V and PI. Data are represented as mean ± S.D. * P < 0.05, ** P < 0.01, one-way ANOVA test. Scale bars, 200 μm (b, d, f, h)