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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Discovery of a natural small-molecule compound that suppresses tumor EMT, stemness and metastasis by inhibiting TGFβ/BMP signaling in triple-negative breast cancer

Fig. 5

ZL170 reduces stemness function in MDA-MB-231 cells. a Immunoblot analyses of Nanog and Sox2 levels in MDA-MB-231 cells that were treated with vehicle or ZL170 at 5, 10 and 20 μM for 48 h (n = 3). b qPCR analysis of Nanog, Sox2, Oct4 and Bmi1 levels in cells that were treated as described in A (n = 3). c and d Flow cytometry analysis of CD49f (c) and CD44 (d) cell-surface expression in MDA-MB-231 cells that were treated with vehicle or ZL170 for 48 h (n = 3). e and f Representative histogram (e) and quantification (f) of ALDH+ subpopulation in MDA-MB-231 cells that were treated with vehicle or ZL170 for 48 h (n = 3). g-j Representative phase-contrast images of vehicle-treated or ZL170-treated MDA-MB-231 cells that were embedded in 3D Matrigel (g) or 3D soft agar gel (i), and tumor sphere number and diameter quantified (h and j). n = 3 independent experiments. Data are represented as mean ± S.D. * P < 0.05, ** P < 0.01, two-sided Student’s t-test. Scale bars = 50 μm (g, i)

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