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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Discovery of a natural small-molecule compound that suppresses tumor EMT, stemness and metastasis by inhibiting TGFβ/BMP signaling in triple-negative breast cancer

Fig. 7

ZL170 inhibits the growth of PyMT-induced breast tumors and reduces their spontaneous metastases to lung. a Growth curves of primary breast tumors from vehicle- and ZL170-treated (i.p., 80 mg/kg/day) MMTV-PyMT transgenic mice (n = 6). b and c Representative H.E. images (b) and quantification (c) of nodules in lungs from the indicated mice (n = 6). d, f and h Immunohistochemical analyses of Ki67 and phospho-histone H3 (D), phospho-Smad2/3 and phospho-Smad5 (f), and Snail, Slug and Nanog (H) in primary tumors of vehicle- and ZL170-treated mice (n = 6). e, g and i Quantification of Ki67+ and phospho-histone H3+ (e), phospho-Smad2/3+ and phospho-Smad5+ (g), and Snail+, Slug+ and Nanog+ (i) cells in primary tumors of the indicated mice (n = 6). j-l Immunofluorescent analyses of E-cadherin and vimentin (j) and K14 (l) in primary tumors of the indicated mice (n = 6). Quantification of staining intensity of E-cadherin and vimentin is shown (k). (M) Masson’s Trichrome staining and immunofluorescent analyses of CD31, α-SMA and F4/80 (m) in primary tumors of the indicated mice (n = 6). Nuclear, DAPI (blue). (N) Quantification of staining intensity of CD31, α-SMA and F4/80 as shown in m. Data are represented as mean ± S.D. ** P < 0.01, two-sided Student’s t-test. Scale bars = 20 μm (d, f, h, j, l, m)

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