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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner

Fig. 5

TKT translocates to the nucleus and interacts with chromatin in HCC cells via a specific NLS. a Immunofluorescence staining of TKT and G6PD in HCCLM3 cells. Unlike the other enzymes of PPP, TKT showed a significant nuclear localization. b Subcellular localization of GFP-tagged TKT, TKT-Y4A and TKT-K6R mutants in Huh7 (green: TKT; blue: nucleus). c Cytoplasmic and chromatic protein was extracted from stable Huh7 cells with Flag-tagged TKT wild-type, TKT-Y4A, TKT-K6R overexpression vectors, and TKT levels were examined. d Enzymatic activity of TKT wild-type, TKT-Y4A and TKT-K6R in the overexpressing Huh7 cells. e Glucose consumption of TKT wild-type, TKT-Y4A, TKT-K6R and TKT-D155A (*: p < 0.05; **: p < 0.01)

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