Fig. 2

Effect of pemetrexed and icotinib on EGFR and AKT/ERK signaling pathways. After treatment with incremental exposure time of pemetrexed (a), incremental concentration levels of icotinib (b) and sequential application of pemetrexed and icotinib (c), the total and phosphorylated EGFR, AKT, and MAPK proteins in PC-9 cells were detected by Western blot. GAPDH served as the loading control. The gray value below protein bands were quantified by Image J software