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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Sequence-dependent synergistic cytotoxicity of icotinib and pemetrexed in human lung cancer cell lines in vitro and in vivo

Fig. 4

The effect of pemetrexed(P) and icotinib(I) on inducing apoptosis of PC-9 cells in vitro. a PC-9 cells were treated with incremental concentration levels of icotinib. b PC-9 cells were treated with incremental concentration levels of pemetrexed. c PC-9 cells were treated with pemetrexed with or without icotinib. The quantitative analysis was shown in the bar graphs. d The release of cytochrome C (cyto-C) was monitored by immunofluorescence analysis in PC-9 cells treated with pemetrexed with or without icotinib (400×). e The levels of the bcl-2, cleaved caspase-3, caspase-9 and PARP proteins were analyzed by Western blot in PC-9 cells treated with or without pemetrexed with or without icotinib. The quantitative analysis was shown in the bar graphs. Data are presented as mean ± SD of triplicate experiments, *p < 0.05

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