Fig. 2

Hsa_circ_0068871 is highly expressed in BCa and exerts oncogenic effects in the BCa cell lines EJ and UMUC3. a and b Hsa_circ_0068871 was highly expressed in tumour tissues compared with adjacent normal tissues (**p < 0.01). c Relative expression of hsa_circ_0068871 in SV-HUC-1 cell and BCa cell lines (*p < 0.05, **p < 0.01, ***p < 0.001). d Expression of hsa_circ_0068871 was confirmed by qPCR in BCa cell lines EJ and UMUC3 transfected with si-NC or si-circ_0068871 (**p < 0.01, ***p < 0.001). e and i Cell proliferation was determined in EJ and UMUC3 cell lines following transfection with circ_0068871, si-NC or si-circ_0068871 (**p < 0.01). f, g and h Cell migration assays were performed in EJ and UMUC3 cells using Transwell chambers (**p < 0.01). j, k and l Wound healing assays were performed in EJ and UMUC3 cells treated with si-NC or si-circ_0068871 (**p < 0.01). m, n and o Colony formation assays were performed in EJ and UMUC3 cells treated with si-NC or si-circ_0068871 (**p < 0.01). p, q and r Cell apoptosis of EJ and UMUC3 after si-NC or si-circ_0068871 as determined by flow cytometry (*p < 0.05, **p < 0.01)