Fig. 4

Hsa_circ_0068871 acts as a sponge for miR-181a-5p, and FGFR3 is a direct target of miR-181a-5p. a and b Putative complementary sites within miR-181a-5p and hsa_circ_0068871 were predicted by bioinformatics analysis (RNA 22v2). c Correlations between hsa_circ_0068871 and miR-181a-5p expression were found with Pearson’s correlation analysis in BCa tissue samples (n = 32). d and e Dual luciferase reporter assays demonstrated that miR-181a-5p is a direct target of hsa_circ_0068871 (**p < 0.01). f miR-181a-5p was pulled down and enriched with hsa_circ_0068871 specific probe and then detected by qRT-PCR (**p < 0.01). g and h Biotin-coupled miR-181a-5p captures hsa_circ_0068871 in the complex compared with biotin-coupled NC in biotin-coupled miRNA capture by agarose gel electrophoresis and analysis products of g by qRT-PCR. i Detection of colocalization of hsa_circ_0068871 and miR-181a-5p in cytoplasm by RNA FISH assay (magnification, × 400). Nuclei were stained blue (DAPI), hsa_circ_0068871 was stained green, and miR-181a-5p was stained red. j Correlations between miR-181a-5p and FGFR3 expression were found with Pearson’s correlation analysis in BCa tissue samples (n = 32). k Putative complementary sites within miR-181a-5p and FGFR3 were predicted by bioinformatics analysis (TargetScan). l Dual luciferase reporter assays demonstrated that FGFR3 is a direct target of miR-181a-5p (**p < 0.01)