Fig. 5

Hsa_circ_0068871 activates STAT3 and regulates the miR-181a-5p/FGFR3 axis. a and d In EJ and UMUC3 cell lines, the expression of miR-181a-5p increased and the expression of FGFR3 decreased after knockdown of hsa_circ_0068871 by qRT-PCR. b and c The protein levels of FGFR3 and p-STAT3 to be decreased after transfection of si-circ_0068871 in EJ and UMUC3 cells by Western blot. e and f The protein levels of FGFR3 and p-STAT3 to be decreased after transfection of miR-181a-5p-mimics in EJ and UMUC3 cell lines by Western blot. g and j Low miR-181a-5p expression partially rescues the promotive effects of hsa_circ_0068871 expression on EJ and UMUC3 cells by CCK-8 assay. h and i, k and l Western blot showed that lowering the expression of miR-181a-5p can partly promote the low expression of FGFR3 and p-STAT3 caused by si-circ_0068871in EJ and UMUC3 cells