Fig. 4

Elevated TRIM44 facilitates cellular EMT in melanoma via the AKT/mTOR pathway. a, Western blot was used to detect levels of p-AKT, AKT, p-ERK1/2, ERK1/2, p-P65, and P65 in the indicated cells. b, Invasion and migration assays were performed in A375-Vector and A375-TRIM44 cells after incubation with either LY-294002 (AKT pathway inhibitor), SC75741 (NF-κB pathway inhibitor), or both. c, Western blot was used to detect levels of TRIM44, EMT markers, p-P65, P65, p-AKT, and AKT in A375-Vector and A375-TRIM44 cells after incubation with LY-294002, SC75741, or both. d, Western blot was used to detect the levels of TRIM44, p-GSK3β, GSK3β, p-mTOR, and mTOR in A2058-shNC, A2058-shRNAs, A375-Vector, and A375-TRIM44 cells. ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001