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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Exosomal transfer of long non-coding RNA SBF2-AS1 enhances chemoresistance to temozolomide in glioblastoma

Fig. 6

XRCC4 is indirectly regulated by lncSBF2-AS1, and SBF2-AS1 depletion induces a delay in DNA damage repair. a XRCC4 protein level in N3T3rd and Rec GBM cells following knockdown of lncSBF2-AS1. GAPDH was used as the loading control. b XRCC4 protein level in N3T3rd and Rec GBM cells following knockdown of lncSBF2-AS1 and/or inhibition of miR-151a-3p. GAPDH was used as the loading control. c Association analysis of the relationship between lncSBF2-AS1 and XRCC4 expression levels in 20 recurrent GBM tissues. d Immunofluorescence staining of γ-H2AX foci in shSBF2-AS1 or shctrl Rec GBM and N3T3rd cells 8 h after TMZ removal. Data represents means of three independent experiments ± SEM. **P < 0.01. Scale bar, 10 μm. e Comet assay of Rec GBM and N3T3rd cells transduced with shSBF2-AS1 or shctrl at the indicated time after TMZ withdrawal. Scale bar, 50 μm. f Western blot analysis of γ-H2AX expression in Rec GBM and N3T3rd cells transfected with shSBF2-AS1 or shctrl at different time points after TMZ treatment. β-actin was used as the loading control

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