Fig. 3

Cx32 regulates the expression of Bcl-2 family proteins in HCC cell lines. a. Cx32 expression was knocked down in HepG2 cells by siRNA transfection. siCx32_2 showed the greatest efficiency in reducing Cx32 expression. b. Transient plasmid transfection into SMMC-7721 cells induced Cx32 overexpression. c. Silencing Cx32 expression in HepG2 cells increased the expression levels of Bax and Bak but decreased the expression level of Bcl-2 (n = 3). ^**, P < 0.01 versus NC. d. Overexpression of Cx32 in SMMC-7721 cells caused the upregulation of Bcl-2 expression and the downregulation of Bax and Bak expression (n = 3). ^**, P < 0.01 versus Vector