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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Cx32 exerts anti-apoptotic and pro-tumor effects via the epidermal growth factor receptor pathway in hepatocellular carcinoma

Fig. 5

Overexpressed Cx32 exerts an anti-apoptotic effect on SMMC-7721 cells in a GJ-independent manner. a. When GJ function was inhibited by pretreatment with 2-APB, overexpression of Cx32 alleviated the SN-induced increase in the levels of cleaved-caspase3 and cleaved-PARP in SMMC-7721 cells, as revealed by western blot analysis (n = 3). **, P < 0.01; ##, P < 0.01. b. Upregulation of Cx32 expression suppressed SN-induced apoptosis in SMMC-7721 cells when GJ function was inhibited by 2-APB, as revealed by flow cytometry (n = 3). **, P < 0.01 versus Cx32 + 2-APB + SN. c. When GJ were physically eliminated by low-density culture conditions, Cx32 overexpression reduced SN-induced increase in the levels of cleaved-caspase3 and cleaved-PARP in SMMC-7721 cells (n = 3). **, P < 0.01; ##, P < 0.01. d. Upregulation of Cx32 expression inhibited SN-induced apoptosis in SMMC-7721 cells in low-density culture, as revealed by flow cytometry (n = 3). **, P < 0.01 versus Cx32 + SN

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