Fig. 8

Overexpression of Cx32 promotes the proliferation of SMMC-7721 cells and protects cells from SN-induced apoptosis in vivo. a. Representative images of the nude mouse xenograft model. b. Tumor growth curves. Overexpression of Cx32 promoted the tumor growth in nude mice and significantly reduced the growth suppression mediated by intragastric injection of SN (0.5 mg/kg). c. Representative images of tumors from the sacrificed nude mice. d. Representative images of IHC for Cx32, EGFR and Src in tumors generated from SMMC-Vector and SMMC-Cx32 cells. Scale bars: 50 μm. e. Overexpression of Cx32 inhibited the SN-induced increase in the levels of cleaved-caspase3 by increasing the levels of EGFR and Src. ^##, P < 0.01 vs Vector +SN. ^**, P < 0.01 versus Vector. f. Representative images of IHC for cleaved-caspase3 in each group. Overexpression of Cx32 inhibited the SN-induced increase in the level of cleaved-caspase3. Scale bars: 50 μm