Fig. 4

TRIM47 promotes metastasis of colorectal cancer cells in vitro and in vivo. a-c Transwell invasion assay was performed in HCT116 and HT29 cells transfected with control siRNA and TRIM47 siRNAs (n = 3, non-parametric Mann-Whitney test, P < 0.05). d Transwell invasion assay was performed in SW480 cells transfected with vector plasmids and TRIM47 overexpression plasmids (n = 3, non-parametric Mann-Whitney test, P < 0.05). e-g Metastasis related proteins Snail, MMP9 and CDH1 were detected in HCT116, HT29 and SW480 cells by western blot (n = 3). h Survival analysis in nude mice bearing colorectal cancer cells injected with PBS, control shRNA adenovirus and TRIM47 shRNA adenovirus, respectively (n = 8, Log-rank test, P < 0.05). i Representative HE staining and summarized data on tumor lung foci in nude mice at 14 weeks after treatment with PBS, control shRNA adenovirus and TRIM47 shRNA adenovirus (n = 8, non-parametric Mann-Whitney test, P < 0.05). j Metastasis related proteins Snail, MMP9 and CDH1 were detected in mice tumor tissue by western blot (n = 3)