Fig. 6

TRIM47 overexpression promotes 5′-fluorouracil resistance of colorectal cancer cells in vitro and in vivo. a and b Cell proliferation assay was performed in SW480 cells transfected with vector plasmids, TRIM47 overexpression plasmids and SMAD4 overexpression plasmids (n = 3, non-parametric Mann-Whitney test, P < 0.05). c Cell transwell invasion assay was performed in SW480 cells transfected with vector plasmids, TRIM47 overexpression plasmids and SMAD4 overexpression plasmids (n = 3, non-parametric Mann-Whitney test, P < 0.05). d Cell proliferation assay was performed in SW480 cells treated with increasing concentrations of 5-FU. (n = 3, non-parametric Mann-Whitney test, P < 0.05). e Cell proliferation assay was performed in SW480 cells transfected with vector plasmids, TRIM47 overexpression plasmids and 5-FU (n = 3, non-parametric Mann-Whitney test, P < 0.05). f Representative images of tumors in nude mice bearing colorectal cancer cells treated with control adenovirus, TRIM47 overexpression adenovirus, 5-FU, TRIM47 overexpression adenovirus & 5-FU (n = 8). g Tumor volume was measured after different treatments in nude mice (n = 8, non-parametric Mann-Whitney test, P < 0.05). h Tumor weight was measured in nude mice after different treatments (n = 8, non-parametric Mann-Whitney test, P < 0.05). i A schematic model of TRIM47 function in colorectal carcinogenesis. TRIM47 interacts with SMAD4 and promotes the ubiquitination and degradation of SMAD4, impairing the suppression of CCL15 and CCR1, ultimately promoting colorectal cancer cells proliferation and metastasis