Fig. 4

Autophagy inhibitors upregulated PD-L1 expression through NF-κB activation. a The effect of pharmacological inhibitors of autophagy on the protein levels of p65, p-p65, IκBα, p-IκBα, IKKα/β and p-IKKα/β was determined by Western blots in NCI-n87 cells. b NCI-n87 cells were treated with or without 3-MA and Baf for 24 h in the absence or presence of BMS-345541, and the levels of PD-L1 protein were examined by flow cytometry. c Knockdown of p65 alleviated the induction of PD-L1 expression at 72 h post-transfection in NCI-n87 cells upon autophagy inhibition by flow cytometry. Results were averaged and blots were representative of 3 independent experiment, *p < 0.05, **p < 0.01