Fig. 5

Inducing effct of lymphocytes and autophagy inhibitors on the expression of PD-L1 by gastric cancer cell lines. a Gating strategy of the cocultures. Gastric cancer cells were gated according to morphology (FSC-A vs. SSC-A) to single cell discrimination (SSC-W vs. SSC-A). The gastric cancer cells were then gated to perform live/dead and lymphocyte discrimination (CD45 vs. 7AAD). These cells were then checked for PD-L1 positivity (PD-L1 vs. 7AAD). The assays on each single sample were repeated at least 3 times. b Evaluation of the expression of PD-L1 in the gastric cancer cell lines AGS or NCI-n87 cocultured with lymphocytes in the presence of chloroquine or 3-MA. Control cells was off drugs for 3 days before the harvest for flowcytometry (Material and Methods). The ratio of PD-L1 MFI minus Isotype control was shown as mean ± S.D. relative to Ctrl from 3 independent experiments, *p < 0.05, **p < 0.01