Fig. 1

LINC00612 is upregulated in bladder cancer (BC) tissues and cell lines. a Heat map with hierarchical clustering of the top 60 differentially expressed lncRNAs between BC samples and normal samples (> 1.5-fold; P < 0.05). b LncRNAs (with fold change > 1.5 and P < 0.05) plotted as a volcano plot. c RT-qPCR was performed to validate LINC00612 expression in BC samples and normal samples (n = 13 vs. n = 8, respectively). ***P < 0.001. d In situ hybridization detection of LINC00612 in BC tissue and normal tissue (× 100 and × 400). e RT-qPCR was performed to measure the relative expression of LINC00612 in BC cell lines (5637, UMUC3 and T24) and human bladder epithelium immortalized cells (SV-HUC-1). The majority of LINC00612 was located in the cytoplasm, according to the nuclear mass separation assay (f) and fluorescence in situ hybridization (g)