Fig. 8

Tumors were collected from nude mice injected with T24 cells transfected with siNC, siLINC00612, miR-590 inhibitor + siNC, miR-590 inhibitor + siLINC00612 (a), and siNC, siLINC00612, Lv-PHF14 + siNC, Lv-PHF14 + siLINC00612 (b). Tumor volume was analyzed every 5 days. Tumor weight was measured 30 days after the tumor transplantation. The differences in tumor volume and mass between the co-transfecting group (siLINC00612 + miR-590 inhibitor and siLINC00612 + Lv-PHF14) and the negative control group were not statistically significant. *P < 0.05, compared with the NC group. c IHC detection of PHF14 in paraffin-embedded tissue sections. d Possible molecular mechanisms of the LINC00612/miR-590/PHF14 axis in bladder cancer: LINC00612 competitively binds to miR-590, which could directly combine with PHF14. PHF14 could enhance the proliferation and invasion ability of bladder cancer cells by activating the WNT pathway via the promotion of migration of β-catenin from the cytoplasm into the nucleus. LINC00612 weakens the inhibiting effect of miR-590 on PHF14