Fig. 1

SNH beneficially reduces EMT progression in NSCLC cells. a. Chemical structure of SNH. b. CCK-8 proliferation assay of A549, NCI-H460 and NCI-H1299 cells treated with different concentrations of SNH for 24 H, 48 H or 72 H. c. Wound scratch assay of A549 and NCI-H1299 cells treated with SNH for 24 H. d. Transwell invasion assays showed that SNH decreased the invasion ability of A549 and NCI-H1299 cells. e. mRNA level of EMT markers in SNH-treated A549 and NCI-H1299 cells as determined by qRT-PCR. f. Expression of EMT markers in SNH-treated A549 and NCI-H1299 cells as determined by western blot analysis. g. Immunofluorescence staining of E-cadherin expression in SNH-treated A549 and NCI-H1299 cells. The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001