Fig. 3

Linc00668 is required for the EMT in NSCLC cells. a. Linc00668 expression in SNH-treated A549 and NCI-H1299 cells after p-Linc00668 transfection as determined by qRT-PCR. b-c. Transwell invasion/migration assays showed that the metastatic ability of A549 and NCI-H1299 cells was increased after p-Linc00668 transfection but was diminished after the addition of SNH. d. mRNA levels of EMT markers in SNH-treated A549 and NCI-H1299 cells after p-Linc00668 transfection as determined by qRT-PCR. e-f. Expression of EMT markers in SNH-treated A549 (e) and NCI-H1299 (f) cells after p-Linc00668 transfection as determined by western blot analysis. g. Immunofluorescence staining of E-cadherin expression in SNH-treated A549 and NCI-H1299 cells after p-Linc00668 transfection. The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001