Fig. 4

Linc00668 functions as a ceRNA and sponges miR-147a in NSCLC cells. a. RIP assays of Linc00668 binding to Ago2 in A549 and NCI-H1299 cell extracts. b. Dual-luciferase reporter assays were used to determine the interaction between miRNAs and Linc00668. c. RIP assays of miR-147a binding to Ago2 in A549 and NCI-H1299 cell extracts. d. Predicted binding sites for miR-147a in the linc00668 sequence. e. RIP assays of Linc00668 binding to Ago2 in miR-147a mimic-transfected A549 and NCI-H1299 cell extracts. f. MiR-147a expression in p-Linc00668-transfected A549 and NCI-H1299 cells as determined by qRT-PCR. g. MiR-147a expression in sh-Linc00668–1-transfected NCI-H1299 cells as determined by qRT-PCR. h-i. Transwell invasion/migration assays showed that the metastatic ability of A549 (h) and NCI-H1299 (i) cells was diminished after miR-147a mimic transfection. j. MiR-147a expression in SNH-treated A549 and NCI-H1299 cells as determined by qRT-PCR. k. mRNA levels of EMT markers in miR-147a mimic-transfected A549 and NCI-H1299 cells as determined by qRT-PCR. L. Expression of EMT markers in miR-147a mimic-transfected A549 and NCI-H1299 cells as determined by western blot analysis. The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001