Fig. 6

Linc00668 regulated Slug expression indirectly via sponging miR-147a. a. Predicted binding sites for miR-147 on Linc00668 and a diagram depicting the construction of the wild type (WT) and mutant type (MUT) pmirGLO-Linc00668 plasmids. b-d. 293 T (b), A549 (c) and NCI-H1299 (d) cells were co-transfected with miR-147a mimics or N.C. mimics and pmirGLO or pmirGLO-Linc00668-WT or pmirGLO-Linc00668-MUT. Luciferase activity was detected 24 H after transfection using a dual-luciferase assay. e. Predicted binding sites for miR-147 on slug and a diagram depicting the construction of the WT and MUT pmirGLO-slug plasmids. f-h. 293 T (f), A549 (g) and NCI-H1299 (h) cells were co-transfected with miR-147a mimic or N.C. mimics and pmirGLO or pmirGLO-slug-WT or pmirGLO-slug-MUT. Luciferase activity was detected 24 H after transfection using a dual-luciferase assay. The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001