Fig. 1

XPR1 overexpression correlates with progression and poor prognosis in TSCC. (a) Real-time PCR analysis of XPR1 mRNA in 8 paired human TSCC tissues (T) and the matched adjacent noncancerous tissues (ANT). Expression was normalized to GAPDH. (b) Western blot analysis of XPR1 protein in 8 paired TSCC tissues and controls. α-Tubulin was used as a loading control. (c) Representative images of XPR1 staining in 4 normal tongue tissues and 128 TSCC patient specimens, which was scored as negative 0, weak + 1, moderate + 2 and strong + 3. The number of each staining score was indicated (brackets). (d) Distribution and comparation between XPR1 staining and T classification or N classification. χ2 test was used. (e) Kaplan–Meier overall survival curve for TSCC patients stratified by low and high XPR1 expression (n = 128, log-rank test). HR, hazard ratio