Fig. 2

Overexpression of XPR1 promotes aggressiveness of TSCC cells in vitro. (a) TSCC cell lines SCC-25 and CAL-27 were stably transduced with XPR1. Overexpression of XPR1 was validated by western blot analysis. α-Tubulin was used as a loading control. (b) MTT assay of indicated cells. (c) Representative images and statastic analysis of colonies formed by indicated cells. (d) Representative micrographs and quantification of the invasiveness of XPR1-overexpressing cells in the transwell matrix invasion assay compared to vector control cells. (e) Flow cytometry analysis of annexin V-FITC/PI staining of the indicated cells treated with cisplatin (20 μM) for 24 h. **P < 0.01, ***P < 0.001