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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Xenotropic and polytropic retrovirus receptor 1 (XPR1) promotes progression of tongue squamous cell carcinoma (TSCC) via activation of NF-κB signaling

Fig. 3

Silencing of XPR1 reduced TSCC aggressiveness in vitro. (a) TSCC cell lines SCC-25 and CAL-27 were stably transduced with two XPR1 shRNAs. Knockdown of XPR1 was validated by western blot analysis. α-Tubulin was used as a loading control. (b) MTT assay of indicated cells. (c) Representative images and statastic analysis of colonies formed by indicated cells. (d) Representative micrographs and quantification of the invasiveness of XPR1-silenced cells in the transwell matrix invasion assay compared to scramble control cells. (e) Flow cytometry analysis of annexin V-FITC/PI staining of the indicated cells treated with cisplatin (20 μM) for 24 h. Quantification was shown below. *P < 0.05, **P < 0.01, ***P < 0.001

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