Fig. 2

P53 mutation is correlated with the high expression of GTSE1. a GTSE1 mRNA expression in the breast cancer tissues and normal breast tissues detected by qPCR. GTSE1 mRNA expression level was standardized to the expression level of housekeeping gene GAPDH. b GTSE1 protein expression level in six paired breast tumor tissues (T) and their adjacent normal tissues (N). c Breast cancer tissues containing 64 non-TNBC and 90 TNBC were stained with GTSE1 antibodies. Representative images of immunohistochemistry (IHC) staining were shown. d GTSE1 protein expression level was higher in TNBC compared to non-TNBC. e GTSE1 mRNA expression level was higher in TNBC compared to non-TNBC based on the bc-GenExMiner v4.1 database. f Real-time PCR analysis of GTSE1 mRNA expression level in normal breast epithelium cell line MCF10A and breast cancer cell lines. g Western blotting of GTSE1 and Ki67 expression in normal breast epithelium cell and breast cancer cell lines. h Based on Oncomine database, the expression level of GTSE1 was higher when p53 was mutated than that of wild type p53. i and j Based on the bc-GenExMiner v4.1 database, the GTSE1 mRNA expression level was positively correlated with MKI67. r = 0.57, p < 0.0001, n = 5510. GAPDH was tested as a loading control in the immunoblotting. Repeating the experiment three times independently