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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner

Fig. 5

GTSE1 promotes breast cancer metastasis by regulating EMT. a and b Silencing GTSE1 could restrain the migratory and invasive ability in MDA-MB-231 cells compared with siRNA negative control cells. d and e Silencing or overexpression of GTSE1 distinctively changed cell migratory ability as detected by wound scratch assay. Yellow lines signify the wound margins. c and f Columns, average of three independent experiments; Bars, S.D. *p < 0.05, **p < 0.01. g Stably transfect-GTSE1 knocked-down cells (SCR, shGTSE1) were injected in mice via the tail vein, the lung of mice was washed by 0.9% NaCl. h The wet lung weight was recorded. i Lung sections were stained with hematoxylin and eosin. j The numbers of microscopic metastatic nodules in the sections were counted. Data were presented as the mean ± S. D, n = 5 per group. k The protein levels of EMT markers were detected by immunoblotting analyses in the indicated cells. l Western blot analysis of the regulators of EMT in indicated cells, and GAPDH as a loading control

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