Fig. 6

GTSE1 can cause multidrug resistance in breast cancer cells. a GTSE1 silencing MDA-MB-231 cells were treated with various concentrations of Taxol, 5-fluorouracil and Adriamycin for 72 h to detect the IC50 using MTS assays. b MDA-MB-231 transiently silenced GTSE and negative control cells were treated with 5-fluorouracil for 72 h, and the total PARP, cleaved PARP, total caspase-3 and cleaved-caspase-3 expression levels were determined by western blotting. GAPDH was tested as the loading control. c and d The indicated MDA-MB-231 and MDA-MB-468 cells were treated with 5-fluorouracil (5 and 0.9μg/ml respectively) for 48 h and test for apoptosis. e and f The indicated MDA-MB-231 and MCF7 cells were treated with 5-fluorouracil (5 and 20μg/ml respectively) for 48 h, then was tested for its apoptosis rate. Three independent experiments were repeated for each assay