Fig. 7

GTSE1 regulates p53 function to alter cell cycle distribution dependent on the mutation state of p53. a and b Indicated cells were collected without drug treatment for flow cytometry assays c and d. Downregulation of GTSE1 resulted in the accumulation of S phase cells by flow cytometry analysis. Indicated cells were stained with PI to analyze cell cycle distribution. Data are presented as means ±SD of three independent experiments. *p < 0.05, **p < 0.01, t-test. e and f Overexpression of GTSE1 results in accumulation of G2 phase cells by flow cytometry analysis. g Western blot analysis of GTSE1, P53, mutant p53, P21, cyclinD1, cyclinE1 expression in indicated cells. GAPDH served as the internal reference. Each experiment was repeated three times. h and i EGF and FGF were added to DMEM/F12 medium for 12–14 days to quantify the number and size of spheres formed by indicating cell lines. j Western blot analysis of Nanog, ABCG2, TAZ, YAP expression in indicated cells. GAPDH served as the internal reference