Fig. 4

ALKBH5-regulated autophagy acts upstream of ULK1 and the PIK3C3-BECN1 complex. a SKOV3 cells were infected with LVRU6P-01, LVRU6P-02, or LVRU6P-NC. A2780 cells were infected with LV121-NC or LV121-ALKBH5. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 72 h, the expression of ATG5, ATG7, Beclin1, Ulk1, and PI3KC3 was detected by WB. b SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01-infected cells were transfected with ATG5 siRNAs. After 48 h, the expression of ATG5 and LC3-II was detected using WB. c SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01 infected cells were transfected with Beclin1 siRNAs. After 48 h, the expression of Beclin1 and LC3-II was detected using WB. d SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01 infected cells were transfected with ATG7 siRNAs. After 48 h, the expression of ATG7 and LC3-II was detected using WB. e SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01 infected cells were transfected with Ulk1 siRNAs. After 48 h, the expression of Ulk1 and LC3-II was detected by WB. f SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01-infected cells were transfected with PI3KC3 siRNAs. After 48 h, the expression of PI3KC3 and LC3-II was detected using WB