Fig. 5
From: ALKBH5 inhibited autophagy of epithelial ovarian cancer through miR-7 and BCL-2
ALKBH5 regulated the cellular proliferation, migration, and autophagy through mTOR pathway. a SKOV3 cells were infected with LVRU6P-01, LVRU6P-02, or LVRU6P-NC. A2780 cells were infected with LV121-NC or LV121-ALKBH5. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 72 h, the expression of mTOR pathway was detected by WB. b A2780 cells were infected with LV121-NC or LV121-ALKBH5. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, Torin1 was added at a concentration of 10 μM. After 8 h, the expression of LC3-II and p-RPS6KB1 was detected by WB