Fig. 6

ALKBH5 regulated EGFR dependent on HuR and miR-7. a We analyzed the correlation between ALKBH5 and HuR using TCGA (http://gepia.cancer-pku.cn/). b SKOV3 cells were infected with LVRU6P-01, LVRU6P-02, or LVRU6P-NC. A2780 cells were infected with LV121-NC or LV121-ALKBH5. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 72 h, the expression of HuR was detected by WB. c Bioinformatics prediction the interaction between HuR and ALKBH5 (https://thebiogrid.org/). d The interaction between HuR and ALKBH5 was confirmed using IP. e-f The expression of miR-7 was determined by qPCR. g The expression of EGFR was detected by WB. h Dual luciferase reporter assay was used to confirm the interaction between miR-7 and EGFR. i We analyzed the correlation between ALKBH5 and EGFR using TCGA (http://gepia.cancer-pku.cn/). jThe expression of EGFR was determined by WB. Error bars represent the standard error. * indicated P < 0.05