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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: ALKBH5 inhibited autophagy of epithelial ovarian cancer through miR-7 and BCL-2

Fig. 8

ALKBH5 regulated ovarian cancer proliferation, invasion, and autophagy flux through Bcl-2. a SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01-infected cells were transfected with pCMV5-Bcl-2. After 48 h, the cell proliferation was assessed by EdU assay. Original magnification, 200X. b SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01-infected cells were transfected with pCMV5-Bcl-2. After 48 h, the invasion assays were performed. c SKOV3 cells were infected with LVRU6P-01 or LVRU6P-NC. After 48 h, puromycin was added at a concentration of 2.5 mg/mL. After 24 h, the LVRU6P-01-infected cells were co-transfected with pCMV5-Bcl-2 and a plasmid encoding mRFP-GFP-LC3. mRFP-GFP-LC3 distribution in SKOV3 cells was analyzed by confocal microscopy after 48 h post-transfection. The LC3 puncta were quantified using Image Pro-Plus 6.0 software. All experiments were repeated three times. The right panel indicates the quantification of LC3 punctate staining. Error bars represent the standard error. *indicated P < 0.05. Scale bar: 50 μm

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