Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Valproic acid exhibits anti-tumor activity selectively against EGFR/ErbB2/ErbB3-coexpressing pancreatic cancer via induction of ErbB family members-targeting microRNAs

Fig. 1

VPA selectively inhibits proliferation/survival of EGFR/ErbB2/ErbB3-coexpressing pancreatic cancer cells. a Western blotting analysis of the expression of EGFR, ErbB2, and ErbB3 in pancreatic cancer cells. b Human pancreatic cancer cells were plated onto 96-well plates with complete culture medium (RPMI1640, 10% FBS). After 24 h, the medium was replaced with control medium (fresh RPMI1640, 0.5% FBS) or same medium containing indicated concentrations of VPA for another 72 h incubation. The percentages of surviving cells from each treatment to controls, defined as 100% survival, were determined by reduction of MTS. c Pancreatic cancer cells were grown in triplicates in the absence or presence of indicated concentrations of VPA for 72 h. The percentages of surviving cells from each group relative to controls, defined as 100% survival, was determined by reduction of MTS following by staining with 0.5% crystal violet for visualization of viable cells. Bars, S.D. All data show the representative of three independent experiments. d HPAF-II and MPanc96 cells were treated with different concentrations of VPA as indicated for 24 h. Cells were harvested and subjected to western blot analyses with specific antibodies directed against Cyclin D1, P21 or β-actin. e, f HPAF-II and MPanc96 cells untreated or treated with different concentrations of VPA as indicated for 24 h were harvested and subjected to western blot analyses with specific antibodies directed against PARP, Cleaved Caspase-3 (C-Casp-3) or β-actin (e), or apoptotic ELISA (f). g, h HPAF-II and MPanc96 cells untreated or treated with VPA (0.5 mmol/L) for 24 h or 48 h were harvested and subjected to western blot analyses with specific antibodies directed against PARP, C-Casp-3 or β-actin (g), or apoptotic ELISA (h). Bars, S.D. All data show the representative of three independent experiments

Back to article page