Skip to main content
Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway

Fig. 2

Increased ATM signaling in cisplatin-resistant NSCLC cells. a Increased ATM expression in cisplatin-resistant NSCLC cells. Total cell extracts were obtained from 549P/ A549CisR and H157P/H157CisR cells. Protein expressions were determined by Western blot analyses using antibodies indicated. b ATM localization in cisplatin-resistant NSCLC cells. Cells (A549P/A549CisR and H157P/ H157CisR) were stained with anti-ATM antibody and then stained with fluorescence- labelled second antibody. c Cisplatin treatment induces EMT in NSCLC cells. A549P and H157P cells were treated with 5 μM cisplatin for indicated time and cell extracts obtained. The expressions of EMT-related genes were determined by Western Blot using antibodies indicated. Represented images were shown. d Increased ATM expression is positively associated with EMT alteration of cisplatin-resistant NSCLC cells. Total cell protein extracts were obtained from parental (A549P and H157P) and cisplatin-resistant cells (A549CisR and H157CisR) and IFC was performed using EMT-related antibodies indicated

Back to article page