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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway

Fig. 3

Inhibition of ATM reverses EMT and decreases invasion ability in cisplatin-resistant NSCLC cells. a-b ATM inhibitor (CP466722) decreased expression in cisplatin-resistant cells determined by Western blot analysis (a) and Real-time PCR analysis (c) Alteration in morphology in in cisplatin-resistant NSCLC cells. A549CisR and H157CisR cells were treated with ATM inhibitor for 24 h and cell morphology was taken under microscope. d The effects of ATM inhibitor on expressions of EMT-related genes in cisplatin-resistant NSCLC cells. A549CisR, H157CisR and parental cells were treated with ATM inhibitor for 24 h. Total cell extracts were obtained and protein expressions were determined by Western blot using antibodies indicated. Presented images were shown. e ATM inhibitor decreases cell invasion in cisplatin-resistant NSCLC cells. Cells indicated were incubated with ATM inhibitor in upper chamber of transwell plates (8 μm pore) and invasive cells to lower chamber were counted under microscope 24 h of incubation. Quantitation of invasion was shown on right panel. f Knockdown of ATM reverses the phenotype of EMT in in cisplatin-resistant NSCLC cells. Total cell extracts were obtained from A549CisR-sc/siATM, H157CisR-sc/siATM and control cells. Protein expressions were determined by Western blot analyses using antibodies indicated. g Alteration of cell morphology. A549CisR-sc/siATM and H157CisR-sc/siATM cells were cultured for 24 h and represented images of cell morphology were shown. h Knockdown of ATM reduced cell invasion. Cells indicated were seeded into upper chamber of transwell plates (8 μM pore) and invasive cells to lower chamber were counted under microscope 24 h after incubation. Quantitation analyses were shown on right Panel. *p < 0.05, **p < 0.01, ***p < 0.001, compared to control cells

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