Fig. 5
ATM up-regulate PD-L1 through JAK1,2/STAT3 pathway in cisplatin-resistant NSCLC cells. a Activation of JAK/STAT3 signaling in cisplatin-resistant NSCLC. Protein expressions of JAK1,2/STAT3 signaling molecules were determined by Western blot. b Cisplatin treatment induces JAK/STAT pathway in NSCLC cells. A549P and H157P cells were treated with 5 μM cisplatin for indicated time and cell extracts obtained. The expressions of JAK1, JAK2, and STAT3 were determined by Western Blot using antibodies indicated. Represented images were shown. c ATM inhibitor decreases mRNA expressions of JAK1, JAK2 and STAT3 determined by qPCR analysis in A549CisR and H157CisR cells. d ATM Knockdown decreases mRNA expression of JAK1,2/STAT3 determined by qPCR in A549CisR-siATM and H157CisR-siATM cells A549CisR. e JAK1,2 inhibitors JAK inhibitor 1 and STAT3 inhibitor stattic downregulates mRNA expressions of PD-L1 determined by qPCR analysis in A549CisR and H157CisR cells (f) ATM overexpression upregulates PD-L1 expression in A549CisR-ATM and H157CisR-ATM cells determined by Western blot. g JAK1,2 inhibitor JAK inhibitor 1 and STAT3 inhibitor stattic downregulates mRNA expressions of PD-L1 determined by qPCR analysis in in A549CisR-ATM and H157CisR-ATM cells. h JAK1,2 inhibitors JAK inhibitor 1 and STAT3 inhibitor stattic reduces cell invasion in cisplatin-resistant cells. A549CisR and H157P/H157CisR cells were seeded in upper chamber of transwell plates (8 μM pore) in the presentation of JAK inhibitor 1 and stattic, 1 × 104) and invasive cells to lower chamber were counted 24 h after the treatment. Quantitation analysis was shown on right panel. i JAK inhibitor 1 and stattic affects mRNA expression of EMT-related genes determined by qPCR in A549CisR and H157CisR cells. *p < 0.05, **p < 0.01, ***p < 0.001, compared to sc cells