Fig. 6

Manipulation of ROS level influenced the efficacy of RvD1 on the expression of COMP and FOXM1. (a and b) CAFs were treated with RvD1, NAC, RvD1 + H₂O₂, and H₂O₂, and the intracellular ROS level was determined by immunofluorescence analysis and flow cytometry analysis using DCF-DA probe. Magnification is × 400, and scale bars = 20 μm. n = three independent experiments, ** P < 0.01 by ANOVA. (c) CAFs were treated with RvD1, NAC, RvD1 + H₂O₂, and H₂O₂ for 48 h, the expression of α-SMA, COMP and FOXM1 were detected by western blotting. n = three independent experiments, ** P < 0.01 by ANOVA. (d) CAFs were treated with RvD1, NAC, RvD1 + H₂O₂, and H₂O₂, and the serection of COMP was detected by Elisa assay. n = three independent experiments, ** P < 0.01 by ANOVA