Fig. 1

G6PD inhibition induces autophagy. a Autophagy analysis on polydatin-treated cells (10–30 μM) performed by immunofluorescence for LAMP1 (top row) and LC3B protein (lower row), 24 h after treatment. b Flow cytometry histograms for the Lysotraker and Lc3B, median fluorescence values are shown in the graphs. Both IF and flow cytometry show an increase of LAMP1, LysoTracker and LC3B fluorescence accordingly to polydatin concentration. c Analysis of puncta on IF for LC3B in polydatin-treated cells (10–30 μM), 24 h after treatment. Puncta represent single autophagosomes. d Immunoblot for LC3B and p62; band densitometry in polydatin treated cells (10–30 μM), 24 h after treatment. LC3BII increases accordingly to polydatin concentration while p62 decreases. p < 0.05, N = 3 (Biological replicates); error bars = SEM