Fig. 2

Analysis of autophagy flux. a IF with Lysotracker and LC3B for autophagy flux analysis on cells treated with polydatin (10 μM), chloroquine (30 μM) and their combination. Co-treatment with chloroquine increases the fluorescence of both LysoTracker and LC3B suggesting that polydatin triggers autophagy. Intensity correlation quotient (ICQ) shows the grade of co-localization of LC3B and Lysotracker; in all three treatments a colocalization grade of about 70% was recorded. b Analysis of puncta for IF of LC3B and Lysotracker on cells treated with polydatin (10 μM), chloroquine (30 μM) and their combination. c Immunoblot with band densitometry for LC3B and P62/sequestosome on cells treated with polydatin (10 μM), chloroquine (30 μM) and their combination. Increase of LC3B and p62 bands in cells co-treated with polydatin and chloroquine confirms that polydatin triggers autophagic flux. *p < 0.05, N = 3; error bar = 95% confidence