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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Glucose-6-phosphate dehydrogenase blockade potentiates tyrosine kinase inhibitor effect on breast cancer cells through autophagy perturbation

Fig. 5

G6PD overexpression confers resistance to lapatinib while its inhibition potentiates its effect. a Viability assay on MCF7mock and MCF7G6PD+ treated with Lapatinib (20 μM) at 24 h and 48 h. IC50 induced by lapatinib at 24 h was 40 μM and 51 μM, respectively, while at 48 h 19.6 μM and 37.6 μM, respectively b Combination index plot from Compusyn software for the analysis of the synergism induced by the combination lapatinib/polydatin. When the points are located below the threshold (1.0), it suggests that the drugs act synergistically. At both 24 and 48 h for the beginning of the treatment, polydatin and lapatinib show a significant synergism. c Annexin V/PI flow cytometry analysis to determine apoptosis in lapatinib and polydatin/lapatinib-treated cells. Drug combination is more effective on both MCF7mock and MCF7G6PD+ if compared to single treatments. The effect on MCF7mock is markedly stronger. d Histograms of the results obtained in Annexin V/PI assay shown in C. e Combination index plot from Compusyn software for the analysis of synergism of the combination lapatinib/polydatin on MDA-MB-231 cell line. The synergism between polydatin and lapatinib is confirmed also on this cell line. * p < 0,05, N = 3 (biological replicates); error bar = SEM

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