Fig. 5
From: Inhibition of EGFR signaling with Spautin-1 represents a novel therapeutics for prostate cancer
Spautin-1 inhibits EGFR signaling pathways in PCa cells. a Western blot analysis was performed to detect the expression of P-EGFR (Y1068), P-EGFR (Y1173) and pan-EGFR in 22Rv1 and PC3 cells treated with Spautin-1 for 24 h. b The expression of P-EGFR (Y1068), P-EGFR (Y1173) and pan-EGFR in 22Rv1 and PC3 cells exposed to Spautin-1 (20 μM) or Gefitinib (2 μM) for 24 h were detected using western blot. c Western blot analysis was performed to detect the expression of P-MKK4, MKK4, P-MEK1/2, MEK1/2 P-EGFR(Y1068), P-EGFR(Y1173) and pan-EGFR in 22Rv1 and PC3 cells exposed to Spautin-1 (20 μM) for the indicated duration