Fig. 6

Spautin-1 inhibits cell survival in glucose deprivation condition via down-regulating Glut1. a) Western blot analysis and b immunofluorescence microscopy were performed to detect the expression of Glut1 in PCa cells treated with Spautin-1 for 24 h. c Cell viability assay was performed on PCa cells exposed to Glut1 siRNA or control siRNA for 48 h in the presence or absence of glucose. d Western blot analysis was performed to detect the expression of Glut1 in PCa cells exposed to Glut1 siRNA or control siRNA for 48 h. e Cell viability assay was performed on PCa cells exposed to Spautin-1 (10 μM) for 24 h in the presence or absence of glucose. f Cell death assay was performed on PC3 cells with or without Glut1 overexpression and exposed to Spautin-1 (10 μM) for 24 h in the presence or absence of glucose. Representative images from three independent experiments are shown. g Pooled data of f are shown. ***p < 0.001 vs. the glucose deprivation control group, ^###p < 0.001 vs. the Spautin-1 combined with glucose deprivation group