Fig. 8

Spautin-1 enhances the sensitivity of PCa cells to Enzalutamide. a, b Cell viability assays were performed on PCa cells exposed to Spautin-1 in the presence or absence of Enzalutamide. *P<0.05 versus single drug treatment. c-g 22Rv1 cells were grafted into BALB/c nude mice and the mice were treated with Spautin-1 (20 mg/kg/d), Enzalutamide (25 mg/kg/d) or Spautin-1 in combination with Enzalutamide for one month. c Shown are xenograft images. d Tumor size, e tumor weight and f body weight are shown. *P<0.05. g Immunohistochemistry staining assay was performed to detect the protein expression of Ki67 of indicated xenografts. Representative images are shown. Scale bar = 50 μm. h A proposed model of Spautin-1 induced cell growth inhibition in PCa