Fig. 2

CDDP-resistant NSCLC promoted M2 polarization in TAMs. a CDDP-resistant NSCLC cells promoted M2 polarization in THP-1 cells in vitro. The insert depicts the co-culture setup. Real-time PCR analysis demonstrated that macrophages co-cultured with A549R and H460R showed a significantly higher M2 marker (CCL22 and TGF-β) as compared to their parental NSCLC cells. b M2 TAMs derived from the co-culture with CDDP-resistant NSCLC cells also showed an increased secretion of M2 cytokines (IL-10 and CCL22) as compared to the TAMs co-cultured with parental NSCLC cells. c Real-time PCR analysis demonstrated the increased post TAM co-culture. An increased mRNA level of IL-4, IL-10 and MIF was observed. **p < 0.01; ***p < 0.001