Fig. 4

Dasatinib-treated NSCLC cells exhibited a lower ability to induce M2 polarization in macrophages. a Real-time PCR profiling of macrophages co-culture with NSCLC cells treated with dasatinib. qPCR results showed that the M2 markers, CCl13 and TGF-β were significantly reduced when co-cultured with H460R and A549R treated with dasatinib (2 μM, 24 h prior to co-culture), while M1 markers, IL-1β and TNF-α remained relatively unchanged. b Comparative ELISA assays of M1 M2 cytokines. Macrophages co-cultured with dasatinib-treated A549R and H460R cells showed a significantly lower secretion of M2 cytokines, IL-10 and CCL22 as compared to their counterparts which were co-cultured with A540R and H460R cells without prior dasatinib treatment. c ELISA assay showed that dasatinib treatment lowered the MIF and IL-6 secreted by both A549R and H460R cells