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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MAPKAPK2 plays a crucial role in the progression of head and neck squamous cell carcinoma by regulating transcript stability

Fig. 5

MK2 regulates the transcript stability of genes involved in HNSCC pathogenesis. CAL27-MK2KD cells treated with ActD were analyzed for the transcript levels of various genes by performing qRT-PCR at different time points (0 min, 30 min, 1 h, 2 h, 4 h and 8 h post-ActD treatment). Results of the kinetic study analyzed using linear regression of mRNA decay rate have been graphically presented here. Following the transcriptional inhibition with ActD, qRT-PCR was performed and results obtained were used to plot the decay curves and half-lives of (a) p27, TNF-α and VEGF in normoxia (b) p27, TNF-α and MKP-1 in hypoxia. GAPDH serves as an endogeneous control. The graphs showcase that MK2KD increased the stability of p27 and MKP-1 transcripts but decreased the half-life (t1/2) of TNF-α and VEGF transcripts in CAL27 cells. All the qRT-PCR assays were performed in triplicate. The results are expressed as means±standard errors of the mean. *, p < 0.05; **, p < 0.01 and ***, p < 0.001 represent the statistical significance compared with control

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