Fig. 3From: SNHG15 is a bifunctional MYC-regulated noncoding locus encoding a lncRNA that promotes cell proliferation, invasion and drug resistance in colorectal cancer by interacting with AIFa SNHG15 and SNORA9 RNA level after knockdown with the two siRNAs separately or in combination in LoVo cells. b Cell proliferation measured by MTS assay after inhibition of SNHG15 in LoVo cells. c SNHG15 and SNORA9 expression levels in SW620 cells after inhibition of SNHG15 with siRNAs. d Proliferation capacity of SW620 after SNHG15 knockdown. e The results of colony formation assay performed quantified after 10 days of inhibition in LoVo cell line and (f) in SW620 cell line. g Cell-cycle phase distribution of LoVo cells determined by propidium iodide-staining (h) Percentage of apoptotic LoVo cells after staining with annexin V and 7-AAD (i) Number of LoVo cells invading through the membrane under the indicated conditions in the transwell assay. j Invading LoVo cells stained on transwell chambers after 48 h in each condition. The statistical analysis is performed by two-tailed Student’s t-test and graphs shows mean ± SEM of values (*p < 0.05, **p < 0.01, ***p < 0.001)Back to article page